Guidelines for qPCR Reaction Setup and Operation. We offer regular workshops on qPCR theory and practice. Also, we have a slide set which describes both the theory and some practical tips that beginning users will find helpful.
Useful resources for qPCR General Theory. There are many resources for learning about qPCR.
The CFX and CFX Connect instruments use LED light sources.Users can designate up to 6 different primer sets per plate for analysis.All of the instruments detect intercalating dyes such as SYBR Green, which provides a lower cost option utilized by most customers.The CFX 96 is capable of multiplexing up to five different fluorphores providing the capability of multiple TaqMan probes.All instruments use a 96-well plate format for quantitative PCR.
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The CFX 96 instrument has optional software to run and interpret high resolution melt curves for allelic descrimination.qPCR has many applications including absolute and relative gene expression, copy number, miRNA, allelic discrimination, detection of viral or bacterial genomes, and 16S ribosomal genes for metagenomics.ĭetects five colors which is useful for Taqman and other probe-based detection methodsĭetects two colors and is designed for SYBR Green detection which is the most common detection method used at UCR.Thus, qPCR provides a sensitive and accurate determination of relative or absolute mRNA abundance and gene expression compared to RT PCR, which measures mRNA abundance at a fixed time point. At a linear phase of amplification, the detection of PCR products (and their corresponding mRNAs) is limited only by the quantity of template.Following individual or group training, instruments can be operated in the Core for a modest time-based fee.It will help to avoid making common mistakes that can be time consuming and very costly. If you are new to this method we strongly advise you to attend. The Genomics Core offers workshops in qPCR theory and practice multiple times per year.We maintain four instruments for real time quantitative PCR To reserve time please go to our Reservation and Billing site. For usage prices please see our Rates page.
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